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Crosstalk between Dental Pulp Stem Cells and Endothelial Cells Augments Angiogenic Factor Expression

Posted on April 10, 2020April 10, 2020 By Taylor No Comments on Crosstalk between Dental Pulp Stem Cells and Endothelial Cells Augments Angiogenic Factor Expression

We aimed to investigate whether the mesenchymal stem cell-endothelial cell crosstalk enhances angiogenic factor expression via nuclear factor kappa B (NF-κB) dependent mechanisms.Human dermal microvascular endothelial cells (HDMECs) and stem cells from human exfoliated deciduous teeth (SHEDs) were co-cultured for 96 h, in the presence of NF-κB decoy oligodeoxynucleotides (ODN) or scramble (control). Vascular endothelial cell growth factor (VEGF) and phospho-NF-κB p65 were measured with enzyme-linked immunosorbent assay. Angiogenesis-related gene expression was analyzed with microarray analysis followed by real-time polymerase chain reaction. Tube formation assay was conducted in the presence of NF-κB decoy.The VEGF and phospho-NF-κB p65 levels were significantly higher in the co-culture with NF-κB decoy scramble than in single-culture and co-culture with NF-κB decoy ODN. Microarray analysis of SHEDs and HDMECs with NF-κB decoy scramble showed higher expression of proangiogenic genes, Bcl-2, NF-κB1, VEGFA, CXCL8, and CXCR1 and lower expression of proapoptotic genes, Bax and Caspase 9, compared to cells with NF-κB decoy ODN. Real-time PCR results for Bcl-2 and CXCL8 showed a similar trend. Tube formation assay showed more tube development in the presence of NF-κB decoy scramble.The SHED-HDMEC crosstalk enhanced proangiogenic factor expression via NF-κB dependent pathways.

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